Emerging technologies / Technologies naissantes Real-time PCR and its application for rapid plant disease diagnostics

  title={Emerging technologies / Technologies naissantes Real-time PCR and its application for rapid plant disease diagnostics},
  author={Norman W. Schaad and Reid D. Frederick},
Rapid-cycle real-time polymerase chain reaction (PCR) methods may revolutionize the manner in which plant pathogens are identified and diseases are diagnosed. As the genomics age progresses and more and more DNA sequence data become available, highly specific primers and fluorescent probe sequences can be designed to yield target amplicons to unique regions of a pathogen’s genome. Portable real-time PCR instruments described here are now allowing for diagnostic assays to be run directly in the… CONTINUE READING
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