• DOI:10.1126/science.1126439
Corpus ID: 43287314

Emergent Properties of Reduced-Genome Escherichia coli

@article{Posfai2006EmergentPO,
  title={Emergent Properties of Reduced-Genome Escherichia coli},
  author={G. Posfai and Guy Plunkett and T. Feh{\'e}r and D. Frisch and G. Keil and Kinga Umenhoffer and V. Kolisnychenko and B. Stahl and S. S. Sharma and M. de Arruda and V. Burland and S. W. Harcum and F. Blattner},
  journal={Science},
  year={2006},
  volume={312},
  pages={1044 - 1046}
}

• G. Posfai, Guy Plunkett, +10 authors F. Blattner
• Published 2006
• Biology, Medicine
• Science

With the use of synthetic biology, we reduced the Escherichia coli K-12 genome by making planned, precise deletions. The multiple-deletion series (MDS) strains, with genome reductions up to 15%, were designed by identifying nonessential genes and sequences for elimination, including recombinogenic or mobile DNA and cryptic virulence genes, while preserving good growth profiles and protein production. Genome reduction also led to unanticipated beneficial properties: high electroporation… Expand

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