Emergent Properties of Reduced-Genome Escherichia coli

@article{Psfai2006EmergentPO,
  title={Emergent Properties of Reduced-Genome Escherichia coli},
  author={Gy{\"o}rgy P{\'o}sfai and Guy Plunkett and Tam{\'a}s Feh{\'e}r and David A. Frisch and G{\"u}nther M. Keil and Kinga Umenhoffer and Vitaliy Kolisnychenko and Buffy Stahl and Shamik S Sharma and Monika de Arruda and Valerie Burland and Sarah W. Harcum and Frederick R. Blattner},
  journal={Science},
  year={2006},
  volume={312},
  pages={1044 - 1046}
}
With the use of synthetic biology, we reduced the Escherichia coli K-12 genome by making planned, precise deletions. The multiple-deletion series (MDS) strains, with genome reductions up to 15%, were designed by identifying nonessential genes and sequences for elimination, including recombinogenic or mobile DNA and cryptic virulence genes, while preserving good growth profiles and protein production. Genome reduction also led to unanticipated beneficial properties: high electroporation… 

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