Emergence of mutation clusters in the HCV genome during sequential viral passages in Sip-L expressing cells.

@article{Hwang2005EmergenceOM,
  title={Emergence of mutation clusters in the HCV genome during sequential viral passages in Sip-L expressing cells.},
  author={Der-Ren Hwang and Hsin-Yu Lai and Ming-Ling Chang and John T. A. Hsu and Chau-Ting Yeh},
  journal={Journal of virological methods},
  year={2005},
  volume={129 2},
  pages={
          170-7
        }
}
Sip-L, a member of the Cupin superfamily, is a hepatic factor capable of supporting hepatitis C virus (HCV) replication in an otherwise non-permissive cell line. HCV-positive serum was used to infect Huh-7 and 293 cells stably expressing Sip-L. Using the culture medium of the infected cells as an infection source, sequential viral passages were carried out in both cell lines. Efficient viral passage was observed in 293-Sip-L cells but not in Huh-7-Sip-L cells. The viral concentrations in the… CONTINUE READING

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