Embryonic stem cells in scaffold-free three-dimensional cell culture: osteogenic differentiation and bone generation

@inproceedings{Handschel2011EmbryonicSC,
  title={Embryonic stem cells in scaffold-free three-dimensional cell culture: osteogenic differentiation and bone generation},
  author={J{\"o}rg GK Handschel and Christian D Naujoks and Rita Antonia Depprich and Lydia Lammers and Norbert R. K{\"u}bler and Ulrich Meyer and Hans-Peter Wiesmann},
  booktitle={Head & face medicine},
  year={2011}
}
Extracorporeal formation of mineralized bone-like tissue is still an unsolved challenge in tissue engineering. Embryonic stem cells may open up new therapeutic options for the future and should be an interesting model for the analysis of fetal organogenesis. Here we describe a technique for culturing embryonic stem cells (ESCs) in the absence of artificial scaffolds which generated mineralized miromasses. Embryonic stem cells were harvested and osteogenic differentiation was stimulated by the… CONTINUE READING

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