Embryonic stem cell development in a chemically defined medium.

  title={Embryonic stem cell development in a chemically defined medium.},
  author={Michael V. Wiles and Britt Marie Johansson},
  journal={Experimental cell research},
  volume={247 1},
Vertebrate germ layer development is an intricately interwoven process with the organism operating as an integrated whole. To examine these processes we have used embryonic stem (ES) cell in vitro differentiation in a serum-free, chemically defined medium (CDM). In CDM, ES cells differentiate as embryoid bodies to neuroectoderm with upregulation of pax-6, without commensurate expression of Brachyury. In the presence of Activin A, pax-6 and Brachyury mRNAs are readily detectable, suggestive of… CONTINUE READING


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