Embryonic myosin heavy chain as a differentiation marker of developing human skeletal muscle and rhabdomyosarcoma. A monoclonal antibody study.

@article{Schiaffino1986EmbryonicMH,
  title={Embryonic myosin heavy chain as a differentiation marker of developing human skeletal muscle and rhabdomyosarcoma. A monoclonal antibody study.},
  author={Stefano Schiaffino and Luisa Gorza and Saverio Sartore and Leopoldo Saggin and Modesto Ottaviano Carli},
  journal={Experimental cell research},
  year={1986},
  volume={163 1},
  pages={211-20}
}
Hybridoma cell lines were obtained from the fusion of NS-O myeloma cells with spleen cells of mice immunized with bovine fetal skeletal myosin. A stable hybridoma clone, BF-G6, produced immunoglobulin G1 k antibodies reacting specifically with embryonic-type myosin heavy chains present in fetal but not in neonatal or adult human skeletal muscle, as determined by enzyme immunoassay and immunoblot analysis. Fetal but not adult skeletal muscle fibers were stained by this monoclonal antibody in… CONTINUE READING

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