Embryonic exposure to o,p′‐DDT causes eggshell thinning and altered shell gland carbonic anhydrase expression in the domestic hen

  title={Embryonic exposure to o,p′‐DDT causes eggshell thinning and altered shell gland carbonic anhydrase expression in the domestic hen},
  author={Lena Holm and Alexandra Blomqvist and Ingvar Brandt and Bj{\"o}rn Brunstr{\"o}m and Yvonne Ridderstr{\aa}le and Cecilia Berg},
  journal={Environmental Toxicology and Chemistry},
The mechanism for contaminant‐induced eggshell thinning in wild birds remains to be clarified. It is generally assumed, however, that it results from exposure of the adult laying female. We have reported that embryonic exposure to the synthetic estrogen ethynylestradiol (EE2) results in eggshell thinning in the domestic hen. The objective of this study was to investigate whether eggshell thinning can be induced following in ovo exposure to a bioaccumulating estrogenic environmental contaminant… 

Reproductive and developmental effects of transovarian exposure to o,p′‐DDT in Japanese quails

The results indicated that transovarian exposure to o,p'-DDT could bring about population declines in avian species through loss of fecundity caused by depression of hatchability and dysfunction of the reproductive tract.

Avian eggshell thinning caused by transovarian exposure to o,p'-DDT: changes in histology and calcium-binding protein production in the oviduct uterus.

Reproductive disorders in birds are the most characteristic effects of DDT contamination of wildlife. Experimental exposure of avian eggs to the estrogenic substance o,p'-DDT causes abnormal

Mechanisms of estrogen-induced effects in avian reproduction caused by transovarian application of a xenoestrogen, diethylstilbestrol

Scanning electron microscopy showed that shell thinning was pronounced in the mammillary and cuticular layers of the eggshell, regions where osteopontin proteins are reportedly located, indicating that transovarian exposure to xenoestrogens causes malformation and dysfunction of the oviducts, where calcium regulating molecules could play key roles in eggshell thinning.

Evaluation of the impact of in‐ovo exposure to dicofol on avian reproduction

The results indicate that transovarian exposure, especially to o,p′‐dicofol, could damage avian reproduction mainly through eggshell thinning.

Effects of transovarian exposure to p,p'-DDT and p,p'-DDE on avian reproduction using Japanese quails.

Exposure experiments to the major component of commercial DDT and its persistent metabolite, p,p'-dichlorodiphenyldichloroethylene (DDE), were performed using Japanese quail eggs; the test substances had no significant effects on the morphology and function of the reproductive systems, although the hatchability of treated eggs was reduced at the highest dose.

Reproductive and developmental toxicity in avian species




Embryonic exposure to oestrogen causes eggshell thinning and altered shell gland carbonic anhydrase expression in the domestic hen.

The results support the hypothesis that eggshell thinning in avian wildlife can result from a structural and functional malformation in the shell gland, induced by xeno-oestrogen exposure during embryonic development.

Disrupted carbonic anhydrase distribution in the avian shell gland following in ovo exposure to estrogen

It is proposed that eggshell thinning in avian wildlife could reflect a functional malformation in the shell gland, already induced by xenoestrogen during embryonic development rather than being caused solely by exposure of the adult bird.

DDE-induced eggshell thinning in birds: effects of p,p'-DDE on the calcium and prostaglandin metabolism of the eggshell gland.

  • C. D. Lundholm
  • Biology, Environmental Science
    Comparative biochemistry and physiology. Part C, Pharmacology, toxicology & endocrinology
  • 1997

Reproductive impairment in Japanese quail (Coturnix japonica) after in ovo exposure to o,p'-DDT

Exposure to 2 mg o,p'-DDT/egg (150 µg/g egg) resulted in impaired sexual behaviour, reduced cloacal gland area and lowered plasma testosterone concentration in males and females.

DDE-induced egg-shell thinning: structural and physiological effects in three species.

Shell thinning in avian eggs by environmental pollutants

The Role of DDE, PCB, Coplanar PCB and Eggshell Parameters for Reproduction in the White-tailed Sea Eagle (Haliaeetus albicilla) in Sweden

It is concluded that the major reason for depressed P during the study period was DDE, but that effects also from PCB were largely concealed by the effects from DDE.