Embryonic Stem Cell Conditioned Medium Supports In Vitro Maturation of Mouse Oocytes

@inproceedings{Miraki2017EmbryonicSC,
  title={Embryonic Stem Cell Conditioned Medium Supports In Vitro Maturation of Mouse Oocytes},
  author={Saber Miraki and Aram Mokarizadeh and Omid Banafshi and Vahideh Assadollahi and Mahdad Abdi and Daem Roshani and Fardin Fathi},
  booktitle={Avicenna journal of medical biotechnology},
  year={2017}
}
BACKGROUND This study aimed to investigate the maturation and fertilization rates of immature mouse oocytes using Embryonic Stem Cell Conditioned Medium (ESCM). METHODS Germinal Vesicle (GV) stage oocytes were observed in 120 NMRI mice, aged 4-6 weeks. GV oocytes with or without cumulus cells were subjected to IVM in either ESCM, Embryonic Stem Cell Growth Medium (ESGM), or α-minimum essential medium (α-MEM). After recording the Metaphase II (MII) oocyte maturation rate, the oocytes were… CONTINUE READING

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