Embryogenesis and Plant Regeneration from Isolated Wheat Zygotes.


Wheat zygotes can be mechanically isolated and cultivated to continue their development in vitro. Since each zygote needs to be individually isolated, only relatively few of these cells are available per experiment. To facilitate embryonic growth despite of this limitation, the zygotes are kept within a culture insert placed in a larger dish which itself… (More)
DOI: 10.1007/978-1-4939-3061-6_29