Embryo selection strategy in sibling oocytes: a novel approach to measuring the likelihood of single‑embryo transfer using a mouse embryo transfer model.

@article{Xu2014EmbryoSS,
  title={Embryo selection strategy in sibling oocytes: a novel approach to measuring the likelihood of single‑embryo transfer using a mouse embryo transfer model.},
  author={Fengqin Xu and Rong Liu and Ying Ma and Li Liu and Ju-Yan Liang and Yan Ma},
  journal={Molecular medicine reports},
  year={2014},
  volume={9 5},
  pages={
          1952-8
        }
}
The present study investigated the effect of embryo transfer on post-implantation development using different fertilization approaches in sibling oocyte procedures. C57BL/6, DBA/2, C3H/HeJ and ICR mice were used at 8-10 weeks of age. Mature oocytes were collected, divided into two groups and fertilized using in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). Different numbers of the resulting blastocysts were then transferred into recipients and designated as either: The… 
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Totipotency segregates between the sister blastomeres of two-cell stage mouse embryos
TLDR
The authors' data place constraints on the commonly accepted idea that totipotency is allocated equally between the two-cell stage blastomeres in mice, and suggest an innate origin of concordance and discordance rather than developmental acquisition.

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