Embedded multicellular spheroids as a biomimetic 3D cancer model for evaluating drug and drug-device combinations.

@article{Charoen2014EmbeddedMS,
  title={Embedded multicellular spheroids as a biomimetic 3D cancer model for evaluating drug and drug-device combinations.},
  author={Kristie Charoen and Brian Fallica and Yolonda L. Colson and Muhammad H. Zaman and Mark W. Grinstaff},
  journal={Biomaterials},
  year={2014},
  volume={35 7},
  pages={
          2264-71
        }
}
Multicellular aggregates of cells, termed spheroids, are of interest for studying tumor behavior and for evaluating the response of pharmacologically active agents. Spheroids more faithfully reproduce the tumor macrostructure found in vivo compared to classical 2D monolayers. We present a method for embedding spheroids within collagen gels followed by quantitative and qualitative whole spheroid and single cell analyses enabling characterization over the length scales from molecular to… Expand
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