Elution of Trichoderma reesei cellulase from cellulose by pH adjustment with sodium hydroxide

@article{Otter2004ElutionOT,
  title={Elution of Trichoderma reesei cellulase from cellulose by pH adjustment with sodium hydroxide},
  author={Don E. Otter and Peter A Munro and Gregory K. Scott and Rick Geddes},
  journal={Biotechnology Letters},
  year={2004},
  volume={6},
  pages={369-374}
}
Up to 45% of bound cellulase (Avicelase) could be recovered from crystalline cellulose by pH adjustment with NaOH (0.008–0.01 M) to pH 10.0 (40°C). Enzyme was simultaneously desorbed and inactivated as the pH increased and both events occurred within 1 minute. Enzyme desorption and stability were also temperature dependent and desorption increased to approximately 65% by adding detergents. 

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