Elucidation of basic mechanistic and kinetic properties of influenza endonuclease using chemically synthesized RNAs.

@article{Olsen1996ElucidationOB,
  title={Elucidation of basic mechanistic and kinetic properties of influenza endonuclease using chemically synthesized RNAs.},
  author={David B. Olsen and Fritz Benseler and James L. Cole and Mark W Stahlhut and Robert E. Dempski and Paul L. Darke and Lawrence C Kuo},
  journal={The Journal of biological chemistry},
  year={1996},
  volume={271 13},
  pages={7435-9}
}
Influenza virus utilizes a unique mechanism for initiating the transcription of viral mRNA. The viral transcriptase ribonucleoprotein complex hydrolyzes host cell transcripts containing the cap 1 structure (m7GpppG(2'-OMe)-) to generate a capped primer for viral mRNA transcription. Basic aspects of this viral endonuclease reaction are elucidated in this study through the use of synthetic, radiolabeled RNA substrates and substrate analogs containing the cap 1 structure. Unlike most ribonucleases… CONTINUE READING