Elucidating the structure of cyclotides by partial acid hydrolysis and LC-MS/MS analysis.


We describe here a rapid method to determine the cyclic structure and disulfide linkages of highly stable cyclotides via a combination of flash partial acid hydrolysis, LC-MS/MS, and computational tools. Briefly, a mixture of closely related cyclotides, kalata B1 and varv A purified from Viola yedoensis was partially hydrolyzed in 2 M HCl for 5 min by… (More)
DOI: 10.1021/ac802175r


  • Presentations referencing similar topics