Elsamicin A can convert the Z-form of poly[d(G-C)] and poly[(G-m5C)] back to B-form DNA.

  title={Elsamicin A can convert the Z-form of poly[d(G-C)] and poly[(G-m5C)] back to B-form DNA.},
  author={Eduardo Jim{\'e}nez-Garc{\'i}a and Jos{\'e} Portugal},
  volume={31 46},
The interaction of poly[(G-C)] and poly[d(G-m5C)] with the antitumor antibiotic elsamicin A, which binds to alternating guanine + cytosine tracts in DNA, has been studied under the B and Z conformations. Both the rate and the extent of the B-to-Z transition are diminished by the antibiotic, as inferred by spectroscopic methods under ionic conditions that otherwise favor the left-handed conformation of the polynucleotides. Moreover, elsamicin converts the Z-form DNA back to the B-form. The… 
11 Citations
Tryptophan intercalation in G, C containing polynucleotides: Z to B conversion of poly [d(G-5M C)] in low salt induced by a tetra peptide.
  • M. R. Rajeswari
  • Chemistry, Biology
    Journal of biomolecular structure & dynamics
  • 1996
Binding of a tetra peptide, lysyl tryptophenyl glycyl lysine O-ter butyl ester (KWGK) with duplex forms of G, C containing polynucleotides, Poly [d(G-C)], Poly [d(G-5M C)], Poly (dG), Poly (dC) and
Reversal of the Z- to B-Conformation of Poly(dA-dT)•Poly(dA-dT) Induced by Netropsin and Distamycin A
The results suggest the preference of these AT-specific drugs for the B-form and the inability of Nt and Dst to form a stable complex with the Z-form of poly(dA-dT)•poly( dA- dT).
Kinetics of the salt-induced B- to Z-DNA transition
Abstract The salt-induced B- to Z-DNA conformational transition is a cooperative- and time-dependent process. From a modified form of the logistic equation which describes an equilibrium between two
Interaction of Isoquinoline Alkaloids with Polymorphic DNA Structures
The interaction of berberine, palmatine, and coralyne with the B, Z, and HL form of poly[d(G‐C)] was studied. Berberine and palmatine showed moderate binding to the B form, while coralyne showed
Thermodynamic characterization of the multivalent binding of chartreusin to DNA.
A detailed thermodynamic profile is obtained for the interaction of this multivalent drug, which makes possible a dissection of DeltaG(obs) into the component free energy terms.
Molecular dynamics study of the binding of elsamicin A to DNA.
Molecular mechanics and dynamics were used to examine the conformational flexibility of elsamicin A, an antitumour antibiotic, in aqueous solution and obtained the first three-dimensional model of the el samicin-A-DNA complex.
Modulation of DNA structure formation using small molecules.


Effects of methylation on a synthetic polynucleotide: the B--Z transition in poly(dG-m5dC).poly(dG-m5dC).
  • M. Behe, G. Felsenfeld
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1981
The results suggest that the sequence m5dC-dG, which occurs in eukaryotic DNA, can have a disproportionately large effect on the B--Z transition.
Interaction of drugs with Z-DNA: cooperative binding of actinomycin D or actinomine to the left-handed forms of poly(dG-dC).poly(dG-dC) and poly(dG-m5dC).poly(dG-m5dC) reverses the conformation of the helix.
Investigation of the interaction of actinomycin D and actinomine with poly(dG-dC) under B- and Z-form conditions shows that the conformation at the binding site is right-handed, even though adjacent regions of the polymer have a left-handed conformation.
Facile transition of poly[d(TG)·d(CA)] into a left-handed helix in physiological conditions
It is reported here that the unmodified DNA polymer d(TG)n·d(CA)n readily undergoes a transition to a Z conformation when subjected to unwinding torsional stress in ionic conditions that are close to physiological.
Long-range allosteric effects on the B to Z equilibrium by daunomycin.
The allosteric effects described here may be of importance toward understanding the mechanism by which the drug inhibits DNA replicative events.
Daunomycin inhibits the B ← Z transition in poly d(G-C)
Since daunomycin binds preferentially to alternating purine-pyrimidine sequences, which are the very sequences capable of undergoing the B leads to Z transition, these effects may be an important part of the mechanism by which the drug inhibits transcription and replication.
Inhibition of the B to Z transition in poly(dGdC).poly(dGdC) by covalent attachment of ethidium: kinetic studies.
It is found that ethidium completely dissociates as the reaction proceeds, but at a rate that lags behind the conversion of the polymer to the Z form, leading to the development of biphasic reaction kinetics.
Ethidium binding to left-handed (Z) DNAs results in regions of right-handed DNA at the intercalation site.
Correlation of binding isotherms with circular dichroism (CD) data indicates that the cooperative binding of ethidium under Z-form conditions is associated with a sequential conversion of the polymer from a left-handed to a right-handed conformation.
Selective DNA cleavage by elsamicin A and switch function of its amino sugar group.
The present results indicate that guanine-specific recognition and selective cleavage of DNA by the antitumor antibiotic elsamicin A equipped with an amino sugar and compare these results with cleavage by chartarin and chartreusin antibiotics and found that an acetylation of the amino group on the elsamsicin A sugar portion plays an interesting switch function for the activity of elsamyin A.
Allosteric conversion of Z DNA to an intercalated right-handed conformation by daunomycin.
  • J. Chaires
  • Chemistry, Biology
    The Journal of biological chemistry
  • 1986
Analysis of the binding of the anticancer drug daunomycin to poly under ionic conditions that initially favor the left-handed Z conformation shows that the number of base pairs converted from the Z to the B conformation per bound drug molecule is salt dependent and provides evidence that drug molecules partition into regions of the polymer in the right-handed conformation.