Elimination of internal restriction enzyme sites from a bacterial luminescence (luxCDABE) operon.


The luxA and luxB genes of luminescent bacteria, most frequently from Vibrio spp., are widely used as reporter genes (3,10). The heterodimer (αβ) luciferase enzyme catalyzes oxidation of the reduced flavin mononucleotide (FMNH2) and a long-chain fatty aldehyde, resulting in emission of light. In vitro, dodecanal or nonanal are used as preferred exogenous… (More)


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