Elimination of interfering substances in the presence of detergent in the bicinchoninic acid protein assay.

  • R E Gates
  • Published 1991 in Analytical biochemistry
Substantial time and effort are necessary to eliminate interference due to thiols and reducing sugars in the bicinchoninic acid protein assay when detergents such as sodium dodecyl sulfate (SDS) are present. A method to eliminate these interfering substances based on the binding of proteins to positively charged nylon membranes at pH 8.5 followed by… CONTINUE READING