Elimination of hop latent viroid upon developmental activation of pollen nucleases.

@article{Matousek2008EliminationOH,
  title={Elimination of hop latent viroid upon developmental activation of pollen nucleases.},
  author={Jaroslav Matousek and Lidmila Orctov{\'a} and Josef Skopek and Karel Pesina and Gerhard Steger},
  journal={Biological chemistry},
  year={2008},
  volume={389 7},
  pages={905-18}
}
Hop latent viroid (HLVd) is not transmissible through hop generative tissues and seeds. Here we describe the process of HLVd elimination during development of hop pollen. HLVd propagates in uninucleate hop pollen, but is eliminated at stages following first pollen mitosis during pollen vacuolization and maturation. Only traces of HLVd were detected by RT-PCR in mature pollen after anthesis and no viroid was detectable in in vitro germinating pollen, suggesting complete degradation of circular… CONTINUE READING