Elimination of "hook-effect" in two-site immunoradiometric assays by kinetic rate analysis.

Abstract

Two-site or sandwich immunoradiometric assays (IRMAs) offer theoretical advantages over competitive immunoassay systems for sensitivity, precision, and rapid incubation. The practical realization of these advantages has been limited by the phenomenon of the "high-dose hook effect," such that high concentrations of an analyte give similar responses to those… (More)

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@article{Hoffman1984EliminationO, title={Elimination of "hook-effect" in two-site immunoradiometric assays by kinetic rate analysis.}, author={Katie L. Hoffman and George H Parsons and L J Allerdt and James Mason Brooks and Leigh Miles}, journal={Clinical chemistry}, year={1984}, volume={30 9}, pages={1499-501} }