Elimination of Bean yellow mosaic virus through thermotherapy combined with meristem-tip culture in gladiolus corms

@inproceedings{Nezamabad2015EliminationOB,
  title={Elimination of Bean yellow mosaic virus through thermotherapy combined with meristem-tip culture in gladiolus corms},
  author={Parisa Sharifi Nezamabad and Mina Koohi Habibi and Akbar Dizadji and Siamak Kalantari},
  year={2015}
}
Bean yellow mosaic virus (BYMV, Potyvirus, Potyviridae) causes serious disease in Gladiolus spp. In this work, the possibility of obtaining BYMV free plant material from virus infected gladiolus corms was studied. Thermotherapy, meristemtip culture and combination of both techniques on infected corms/meristem-tip explants (0.5–1 mm in length) resulted in BYMV elimination up to 15.38, 78.04 and 86.66%, respectively, as determined by double antibody sandwich-enzyme-linked immunosorbent assay (DAS… CONTINUE READING

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