Eliminating solid phase extraction with large-volume injection LC/MS/MS: analysis of illicit and legal drugs and human urine indicators in U.S. wastewaters.

Large-volume (1800 microL) injection (LVI) followed by liquid chromatography/tandem mass spectrometry was developed and optimized to eliminate the need for off- and on-line solid phase extraction as a sample preparation step. Centrifugation of raw municipal influent followed by LVI was optimized for the routine determination of illicit drugs and related… (More)