Eliminating malignant contamination from therapeutic human spermatogonial stem cells.

@article{Dovey2013EliminatingMC,
  title={Eliminating malignant contamination from therapeutic human spermatogonial stem cells.},
  author={Serena L Dovey and Hanna Valli and Brian P Hermann and Meena Sukhwani and Julia Donohue and Carlos Arturo Iregui Castro and Tianjiao Chu and Joseph S. Sanfilippo and Kyle E. Orwig},
  journal={The Journal of clinical investigation},
  year={2013},
  volume={123 4},
  pages={1833-43}
}
Spermatogonial stem cell (SSC) transplantation has been shown to restore fertility in several species and may have application for treating some cases of male infertility (e.g., secondary to gonadotoxic therapy for cancer). To ensure safety of this fertility preservation strategy, methods are needed to isolate and enrich SSCs from human testis cell suspensions and also remove malignant contamination. We used flow cytometry to characterize cell surface antigen expression on human testicular… CONTINUE READING
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