Elicitation of Taxus sp. cell cultures for production of taxol

  title={Elicitation of Taxus sp. cell cultures for production of taxol},
  author={Veeresham Ciddi and Venkatesh Srinivasan and Michael L. Shuler},
  journal={Biotechnology Letters},
SummaryThe addition of cell extracts and cultures filtrate of Pencillium minioluteum, Botrytis cinerea, Verticillium dahliae, and Gilocladium deliqucescens on the tenth day after transferring Taxus sp. (RO1-M28) cell suspensions into an induction medium, further improved the production of TaxolTaxol is a registered trademark of Bristol Myers Squibb for paclitaxel. and total taxanes. Arachidonic acid (1mg/L) addition at the time of inoculation increased Taxol production by 150%. Oxidative stress… 
Plant cell culture for production of paclitaxel and other taxanes.
  • J. Zhong
  • Biology, Engineering
    Journal of bioscience and bioengineering
  • 2002
Enhanced paclitaxel production induced by the combination of elicitors in cell suspension cultures of Taxus chinensis
Taxus chinensis suspension cells were cultured in the modified Gamborg's B5 medium. Addition of 50 mg chitosan l−1, 60 μM methyl jasmonate and 30 μM Ag+ resulted in the greatest paclitaxel
Methyl jasmonate stimulates jaceosidin and hispidulin production in cell cultures of Saussurea medusa
It is observed that the activities of phenylalanine ammonia lyase were transiently increased after treatment with MJ, which suggests that this elicitor modifies jaceosidin and hispidulin production by regulating the phenylpropanoid pathway.
Involvement of NO in fungal elicitor-induced activation of PAL and stimulation of taxol synthesis inTaxus chinensis suspension cells
It is indicated that NO, produced via NOS in taxus chinensis cells treated with fungal elicitor, might act as an essential signaling molecule for triggering the activation of PAL and synthesis of taxol.
Oxidative Stress and Taxol Production Induced by Fungal Elicitor in Cell Suspension Cultures of Taxus Chinensis
It is concluded that oxidative stress had the deleterious effect onTaxus chinensis cell suspension cultures with fungal elicitor and ascorbic acid changed the oxidative stress and taxol production.
Production of Taxol and its Analogues from Cell Cultures of Taxus wallichiana
Preursors and growth retardants significantly improved the production of taxol, DAB and BA in cultures grown in production medium and on HPLC analysis using photodiode array detector.


Elicitation of anthocyanin production in cell cultures of carrot (Daucus carota L) by using elicitors and abiotic stress
A cell line of carrot which produces anthocyanin was subjected to various elicitors and abiotic stresses: the elicitors tested were culture filtrates and cell extracts of certain bacteria and yeasts and Ca was the most effective.
Cell Culture of Taxus as a Source of the Antineoplastic Drug Taxol and Related Taxanes
Callus cultures of Taxus cuspidata and Taxus canadensis were induced using different tissue explants including green and red arils, seed contents, young stems and needles to inhibit callus darkening and subsequent growth reduction.
Improved taxol yield by aromatic carboxylic acid and amino acid feeding to cell cultures of taxus cuspidata
Examination of the effect of various concentrations of phenylalanine, benzoic acid, N‐benzoylglycine, serine, glycine, alanine, and 3‐amino‐3‐phenyl‐propionic acid on taxol accumulation in cell suspensions of Taxus cuspidata, cell line FCL1F, and in developing callus cultures finds increases in taxol amounts at stationary phase brought by the most effective precursors.
Differential enhancement of benzophenanthridine alkaloid content in cell suspension cultures of Sanguinaria canadensis under conditions of combined hormonal deprivation and fungal elicitation.
Levels of dopamine, a precursor of the alkaloids, fluctuated over the incubation period, but displayed a 2- to 6-fold increase in cell suspensions grown without hormone, and these increases were enhanced by the addition of fungal elicitor.
Taxol production in bioreactors: Kinetics of biomass accumulation, nutrient uptake, and taxol production by cell suspensions of Taxus baccata
The similarity of kinetics in the three bioreactor configurations suggests that taxol production by T. baccata cell suspensions is amenable to scateup.
High performance liquid chromatographic separation of taxol and related compounds from Taxus brevifolia
Using cyano and phenyl bonded silica gel phases in the reverse phase mode, was have developed sensitive analytical high performance liquid chromatography (HPLC) methods for detecting taxol and four
The taxol supply crisis. New NCI policies for handling the large-scale production of novel natural product anticancer and anti-HIV agents.
In responding to the drug supply crisis which emerged with the demonstration of the clinical efficacy of taxol, the NCI has developed plans to avert similar supply crisis in the future by initiating exploratory research projects for large-scale production of promising agents at the earliest possible point following the demonstrated antitumor activity.