Elevated polysaccharide production by mutants of the fungus Aureobasidium pullulans

  title={Elevated polysaccharide production by mutants of the fungus Aureobasidium pullulans},
  author={Thomas P. West and B. Reed-Hamer},
  journal={Fems Microbiology Letters},
Two mutants of the fungus Aureobasidium pullulans ATCC 42023 were isolated that exhibited elevated polysaccharide production. Both mutants were isolated using a combination of chemical mutagenesis and resistance to growth inhibitors. It was found that both mutants elaborated higher polysaccharide levels after 7 days of growth on corn syrup or sucrose, respectively, compared to ATCC 42023. The dry weights of the mutant cells were found not to differ greatly from those of the parent cells whether… 

Short Communication: Effect of manganese on polysaccharide production and cellular pigmentation in the fungus Aureobasidium pullulans

Manganese supplementation resulted in higher polysaccharide levels and reduced cellular pigmentation by more than 8- or 17-fold after growth for 7d of the fungus Aureobasidium pullulans ATCC 42023 on

Fungal production of the polysaccharide pullulan from a plant hydrolysate

  • T. West
  • Medicine, Biology
    Zeitschrift fur Naturforschung. C, Journal of biosciences
  • 2017
Unlike what has been observed for other biomass substrates, a polysaccharide with a high pullulan content can be produced at a relatively high yield by a fungus grown on a grass hydrolysate indicating thatpullulan could be produced using a biomass-based process.

Polysaccharide production by sponge‐immobilized cells of the fungus Aureobasidium pullulans

It was found that fungal cells grown on corn syrup, sucrose or glucose as a carbon source could be immobilized in sponge cubes and that comparable cell weights and viable cell concentrations were immobilized.

Polysaccharide production by immobilized Aureobasidium pullulans cells in batch bioreactors.

Cells of the fungus Aureobasidium pullulans ATCC 201253 were entrapped within 4% agar cubes or 5% calcium alginate beads and were examined for their production of the polysaccharide pullulan in batch

Exopolysaccharide production by entrapped cells of the fungus Aureobasidium pullulans ATCC 201253

  • T. West
  • Biology, Engineering
    Journal of basic microbiology
  • 2000
The production of the exopolysaccharide pullulan using entrapped cells of the fungus Aureobasidium pullulans ATCC 201253 was investigated and fungal cells entrapping in carrageenan demonstrated a lower degree of leakage than did thoseEntrapped in agarose.

A pullulan‐degrading enzyme activity of Aureobasidium pullulans

A pullulan‐degrading enzyme activity was detected in the culture medium after growth of the fungus Aureobasidium pullulans on corn syrup as a carbon source. The product of pullulan catabolism by this

Production of the Polysaccharide Pullulan by Aureobasidium pullulans Cell Immobilization

  • T. West
  • Biology, Engineering
  • 2022
Carrier-binding techniques may be more effective than entrapment techniques for A. pullulans cell immobilization, since carrier-binding is less likely to affect the pullulan content of the polysaccharide being synthesized.



Polysaccharide produced by the genus Pullularia. I. Production of polysaccharide by growing cells.

A dextranlike polysaccharide was found to be produced on substrates of sucrose, maltose, glucose, and fructose by growing cells of various strains of the genus Pullularia. The polysaccharide,

Flocculant and chemical properties of a polysaccharide from Pullularia pullulans.

Structural studies on the fractions of purified polysaccharide by methylation and by periodate oxidation techniques prove that PP-floc is linear and composed of alpha-(1 --> 4) and alpha-1 --> 6) glucopyranosyl units in the approximate ratio of 2:1.


The nature of this absorption of nystatin by microorganisms and its importance as a factor in the agent's unique specificity for fungi are investigated.

Structural Uniformity of Pullulan Produced by Several Strains of Pullularia pullulans

Extracellular polysaccharides produced by 3 strains of Pullularia pullulans were fractionated by treating with cetyl trimethyl ammonium hydroxide into soluble and insoluble fractions, and the struc...

Enrichment of fungal mutants by selective cell-wall lysis

A new technique based on selective cell-wall lysis is described, which has wide applicability and is highly efficient for isolating mutants differing from the wild-type strain in growth requirements.


A neutral extracellular glucan ([α]D23 + 189°) was produced in 12% yield by Pullularia pullulans (de Bary) Berkhout grown on a glucose substrate. A single symmetrical peak on free-boundary electrop...

The production of polysaccharides by fungi active in the decomposition of wood and forest litter.

  • B. Bernier
  • Environmental Science
    Canadian journal of microbiology
  • 1958
Four fungi known to be active in the decomposition of wood and forest litter were grown on a liquid medium containing salts and glucose and also on aqueous extracts of a variety of fresh leaves and...