Elevated genetic variation within virulence-associated Botrytis cinerea polygalacturonase loci.

@article{Rowe2007ElevatedGV,
  title={Elevated genetic variation within virulence-associated Botrytis cinerea polygalacturonase loci.},
  author={Heather C. Rowe and Daniel J. Kliebenstein},
  journal={Molecular plant-microbe interactions : MPMI},
  year={2007},
  volume={20 9},
  pages={
          1126-37
        }
}
Botrytis cinerea, or gray mold, is a necrotrophic fungal pathogen of hundreds of plant species. The genetic diversity of B. cinerea may contribute to its broad host range; however, the level and structure of genetic variation at pathogenesis-associated loci has not been described. B. cinerea possesses six distinct cell-wall-degrading polygalacturonases (PGs), enzymes of demonstrated importance to pathogenesis and interaction with host plant defenses. Sequencing a collection of 34 B. cinerea… 
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References

SHOWING 1-10 OF 50 REFERENCES
Botrytis cinerea endopolygalacturonase genes are differentially expressed in various plant tissues.
TLDR
The pattern of Bcpg gene expression indicates that B. cinerea is equipped with a flexible enzymatic pectate degradation machinery, and pinpoint new targets for gene disruption studies.
The endopolygalacturonase gene Bcpg1 is required for full virulence of Botrytis cinerea.
TLDR
Results indicate that the Bcpg1 gene, encoding endopolygalacturonase, is required for full virulence in B. cinerea.
Genetic Structure of Botrytis cinerea Populations from Different Host Plants in California.
TLDR
The parsimony tree length permutation (PTLPT) and index of association ( IA) analyses of MP-PCR data set were consistent with absence of sexual recombination in sampled populations of this pathogen.
Cloning and Partial Characterization of Endopolygalacturonase Genes from Botrytis cinerea
TLDR
The cloning and characterization of five additional endoPG-encoding genes from B. cinerea SAS56 resulted in the identification of five monophyletic groups of closely related proteins.
Genetic characterisation of Botrytis cinerea populations in Chile
TLDR
Analysis of isolates collected in Chile from grapes, tomato, kiwifruit and blueberry revealed the existence of genetic differentiation in the authors' sample between isolates from the different host plants, and PCR-RFLP markers showed that isolates sampled from grapes and tomato were genetically differentiated.
Phenotypic Differences Between vacuma and transposa subpopulations of Botrytis cinerea
TLDR
A significant negative correlation was established between virulence and mycelial growth rate, and the grapevine leaves were significantly more susceptible to B. cinerea than those of tobacco.
Two Sibling Species of the Botrytis cinerea Complex, transposa and vacuma, Are Found in Sympatry on Numerous Host Plants.
TLDR
This study confirms that B. cinerea is a complex of sibling species and shows that the sibling species occur sympatrically on many host plants, however, the two species seemed to have different pathogenic behaviors.
RFLP markers show genetic recombination in Botryotinia fuckeliana (Botrytis cinerea) and transposable elements reveal two sympatric species.
TLDR
It is concluded that there are two sympatric populations of B. fuckeliana in Champagne, one of which seems to be local and well adapted, while the other is presumably a heterogeneous migrant population.
Variability of Three Isolates of Botrytis cinerea Affects the Inhibitory Effects of Calcium on this Fungus.
TLDR
Evidence that the biological and statistical variability in research can be affected by the B. cinerea isolate selected is supported, despite this variation, Ca treatment of apples reduced decay caused by all three Botrytiscinerea isolates.
Infection Strategies of Botrytis cinerea and Related Necrotrophic Pathogens
TLDR
The pathogen is a typical necrotroph, inducing host cell death resulting in serious damage to plant tissues, culminating in rot of the plant or the harvested product.
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