Elevated Uptake of Low Density Lipoproteins by Human Lung Cancer Tissue in Vivo1

Abstract

In order to explore new treatment modalities for cancer, it is impor tant to identify qualitative or quantitative differences in metabolic pro cesses between normal and malignant cells. Previous in vitro and in vivo studies have shown that acute myelogenous leukemia cells have elevated receptor-mediated uptake of low density lipoproteins (LDL), compared to normal VVBC. High receptor-mediated uptake of LDL by certain cancer cells in tissue culture and experimental tumors in animals in vivo has also been demonstrated. The present study was undertaken to com pare the in viro assimilation of LDL by human lung cancer tissue with that by surrounding lung tissue. Ten patients with newly diagnosed lung tumors, scheduled for surgery, received an i.v. injection of [l4C|sucroselabeled LDL. Following cellular uptake and degradation of the LDL particle, the radiolabeled sucrose moiety remains trapped in the lysosomal compartment, making this labeling technique useful for in vivo studies of tissue uptake of LDL. Radioactivity was determined in plasma and in tissue biopsies obtained at surgery 1-3 days after injection. The uptake of radioactivity in lung cancer tissue was elevated (1.5-3.0-fold), compared to surrounding tissue, in 7 of 9 patients with primary lung cancer. The most rapid preoperative disappearance of radioactivity from plasma was found in 2 patients with large tumors exhibiting high LDL uptake, relative to normal lung tissue. These findings support the hy pothesis that the selectivity of cytotoxic agents can be enhanced also in nonhematological malignancies by administering the drugs incorpo rated in LDL particles.

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Cite this paper

@inproceedings{Vitols2006ElevatedUO, title={Elevated Uptake of Low Density Lipoproteins by Human Lung Cancer Tissue in Vivo1}, author={Sigurd Vitols and Curt Peterson and Olle Larsson and Peter Holm and Bengt {\~A}•berg}, year={2006} }