Elemental modifications and polycyclic aromatic hydrocarbon metabolism in human fibroblasts

  title={Elemental modifications and polycyclic aromatic hydrocarbon metabolism in human fibroblasts},
  author={Ronald Wilson Hart and F. Bernard Daniel and O R Kindig and Cynthia A. Beach and Laurie B. Joseph and Robert C Wells},
  journal={Environmental Health Perspectives},
  pages={59 - 68}
Mineral fibers and particulates represent one of the best documented, economically important, and ubiquitously occurring categories of human carcinogens. Yet, while a wealth of information exists concerning the mechanism of action of physical, chemical, and viral carcinogens, virtually nothing is known relative to the mechanism of action of this economically important class of carcinogenic compounds known as mineral fibers and particulates. While the length and diameter of various forms of… 
Fiber carcinogenesis: epidemiologic observations and the Stanton hypothesis.
  • J. Harington
  • Medicine
    Journal of the National Cancer Institute
  • 1981
Concern is currently growing about possible human cancer risk posed by other natural or man-made fibers, such as sepiolite, attapulgite, fibrous glass, and fibrous zeolites, including asbestos fiber, which has been associated in some way or another with human disease.
Genotoxic Effects of Natural and Man-made Mineral Fibers
The main purpose was to examine and compare the behavior of these materials at the cellular level, in particular with respect to the mechanisms involved in the process of carcinogenesis, using a well-established cellular model designed for the detection of genotoxic properties.
Interactions of chrysotile and benzopyrene in a human cell culture systems.
The asbestos had no demonstrable influence on the level of B(a)P metabolism during the 24-hr period following simultaneous exposure of asbestos and hyrdocarbons, and there was no strong indication of a perturbation of thelevel of DNA-B( a)P binding following simultaneous administration of increasing levels of asbestos in addition to 1 microM hydrocarbon.
In vitro assessment of asbestos genotoxicity.
  • F. Daniel
  • Biology
    Environmental health perspectives
  • 1983
Asbestos fibers are clearly oncogenic to humans and animals, but, except for clastogenic effects in rodent cells, there is little evidence for genotoxicity of fibers.
Absence of genotoxic effects of nonasbestos mineral fibers
The results point to the immediate necessity of studying more extensively the biological effects of fibrous materials that can be used as substitutes for asbestos.
Interaction of DNA with Silicic Acid
It is reported that degradation of DNA may take place as a result of its interaction with silicic acid in DNA and RNA in asbestos-exposed animals.
Implications of multiple mechanisms of carcinogenesis for short-term testing.
Extra care must be taken to establish a genuine lack of genotoxicity before an agent can be classified (and regulated) as a promoter (lacking the ability to initiate tumor growth but still enhancing tumor development).
Adsorption of Macromolecules on Mineral Fibers
The results suggest that adsorption by mineral fibers may induce changes in enzyme-substrate interactions and therefore could interfere with normal biological processes.


The In Vitro Effects of Mineral Dusts
The identification of dangerous dusts and the industries in which they are produced has traditionally resulted from the clinical recognition of a rare condition in a delineated occupational group, however this "epidemiological" approach has the following disadvantages.
Aryl hydrocarbon hydroxylase in asbestos workers.
Differences in inducibility resulted from increases in induced activity, not from any appreciable change in basal activities, and may bear some relation to the greatly increased risk of bronchogenic carcinoma in asbestos workers who smoke cigarettes.
The biological effects of mineral fibres, especially asbestos, as seen from in vitro and in vivo studies.
Two in vitro models have been extensively used to compare the biological action of different types of asbestos fibres: the haemolytic effect and the cytotoxic one on macrophages grown in cell
Pulmonary Asbestosis III: Carcinoma of Lung in Asbesto-Silicosis
Primary carcinoma of the lung has attained a position of major interest in malignant neoplastic disease, through at least a more common recognition, if not more frequent occurrence, within recent
Putative inhibitory effects of chrysotile, crocidolite, and amosite mineral fibers on the more complex surface membrane glycolipids and glycoproteins
The present studies support the concept that a metabolic rather than immediate masking effect is involved in asbestos treatment, and suggest that the membrane changes induced by asbestos serve to allow other mutagens to pass into the cell so as to act on the nuclear structure.
The cytotoxic effects of asbestos and other mineral dust in tissue culture cell lines.
The cytotoxic effects of 15 dusts have been tested in 2 cell lines and it is suggested that the study of the interactions between cells and dusts in culture may lead to an understanding of the pathogenesis of theseDusts in man and animals.
Mesotheliomata in Rats after Inoculation with Asbestos and Other Materials
The results of four experiments in which SPF Wistar rats were inoculated intrapleurally with asbestos or other materials support the hypothesis that the finer fibres are the more carcinogenic, and this is additional to the known aerodynamic advantage which the fine fibres have in penetrating to the periphery of the lung.
Cellular interactions in fibrogenesis
The mechanisms by which collagen synthesis is controlled are of interest and the results of experiments show that alveolar and other macrophages arise from blood-borne cells, presumably monocytes, but provide no evidence that collagensynthesizing fibroblasts originate in this way.