Electrotransformation of intact cells ofBrevibacterium flavum MJ-233

Abstract

Electroporation allowed transformation of intact cells ofBrevibacterium flavum MJ-233. The two plasmids used for electroporation were pCRY2 (6.3 kilobases) and pCRY3 (8.2 kilobases). Both plasmids contain the chloramphenicol-resistance gene and the autonomous replication origin inB. flavum MJ-233. The efficiency of electrotransformation was optimal with cells harvested at the middle log phase of growth, and was imporved by the addition of 1.0U/ml of penicillin G to the culture medium. The optimum yield of transformants per μg DNA was 5×104 when the cell suspension was pulsed at a cell density of 1×1010/ml and at a DNA amount of 1.0μg.

DOI: 10.1007/BF01573866

7 Figures and Tables

Statistics

050100150'04'05'06'07'08'09'10'11'12'13'14'15'16'17
Citations per Year

61 Citations

Semantic Scholar estimates that this publication has 61 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Satoh1990ElectrotransformationOI, title={Electrotransformation of intact cells ofBrevibacterium flavum MJ-233}, author={Yusuke Satoh and Kazuhisa Hatakeyama and Kazuhiro Kohama and Michihiko Kobayashi and Yasurou Kurusu and Hideaki Yukawa}, journal={Journal of Industrial Microbiology}, year={1990}, volume={5}, pages={159-165} }