Electrotransformation of Thiobacillus ferrooxidans with plasmids containing a mer determinant.

  title={Electrotransformation of Thiobacillus ferrooxidans with plasmids containing a mer determinant.},
  author={Teruo Kusano and Kazuyuki Sugawara and Chihiro Inoue and Toshikazu Takeshima and Masahiko Numata and Takaaki Shiratori},
  journal={Journal of bacteriology},
  volume={174 20},
The mer operon from a strain of Thiobacillus ferrooxidans (C. Inoue, K. Sugawara, and T. Kusano, Mol. Microbiol. 5:2707-2718, 1991) consists of the regulatory gene merR and an operator-promoter region followed by merC and merA structural genes and differs from other known gram-negative mer operons. We have constructed four potential shuttle plasmids composed of a T. ferrooxidans-borne cryptic plasmid, a pUC18 plasmid, and the above-mentioned mer determinant as a selectable marker. Mercury ion… CONTINUE READING
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