Electrostatics significantly affect the stability of designed homeodomain variants.

@article{Marshall2002ElectrostaticsSA,
  title={Electrostatics significantly affect the stability of designed homeodomain variants.},
  author={Shannon A. Marshall and Chantal Smith Morgan and Stephen L. Mayo},
  journal={Journal of molecular biology},
  year={2002},
  volume={316 1},
  pages={
          189-99
        }
}
The role of electrostatic interactions in determining the stability of designed proteins was studied by constructing and analyzing a set of designed variants of the Drosophila engrailed homeodomain. Computational redesign of 29 surface positions results in a 25-fold mutant with moderate stability, similar to the wild-type protein. Incorporating helix dipole and N-capping considerations into the design algorithm by restricting amino acid composition at the helix termini and N-capping positions… CONTINUE READING

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