Electrostatic surface plasmon resonance: direct electric field-induced hybridization and denaturation in monolayer nucleic acid films and label-free discrimination of base mismatches.

@article{Heaton2001ElectrostaticSP,
  title={Electrostatic surface plasmon resonance: direct electric field-induced hybridization and denaturation in monolayer nucleic acid films and label-free discrimination of base mismatches.},
  author={R J Heaton and A. W. Peterson and Rosina M. Georgiadis},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2001},
  volume={98 7},
  pages={3701-4}
}
We demonstrate that in situ optical surface plasmon resonance spectroscopy can be used to monitor hybridization kinetics for unlabeled DNA in tethered monolayer nucleic acid films on gold in the presence of an applied electrostatic field. The dc field can enhance or retard hybridization and can also denature surface-immobilized DNA duplexes. Discrimination between matched and mismatched hybrids is achieved by simple adjustment of the electrode potential. Although the electric field at the… CONTINUE READING

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