Electrostatic orientation of enzymes on surfaces for ligand screening probed by force spectroscopy.

@article{Wang2006ElectrostaticOO,
  title={Electrostatic orientation of enzymes on surfaces for ligand screening probed by force spectroscopy.},
  author={Xiuzhu Wang and Dejian Zhou and Kumar Sinniah and Caroline Clarke and Louise Birch and Haitao Li and Trevor Rayment and Chris Abell},
  journal={Langmuir : the ACS journal of surfaces and colloids},
  year={2006},
  volume={22 3},
  pages={887-92}
}
In this letter, we show that electrostatic immobilization provides a simple but effective approach for the immobilization and orientation of carbonic anhydrase onto charged surfaces. The enzyme is oriented differently on oppositely charged surfaces, with the majority of active sites facing upward on a positively charged surface and downward on a negatively… CONTINUE READING