Electrostatic immobilization of glucose oxidase in a weak acid, polyelectrolyte hyperbranched ultrathin film on gold: fabrication, characterization, and enzymatic activity.

Abstract

In this paper we show that hyperbranched polymers can be used as a host matrix for electrostatic entrapment of enzymes. Specifically, amine-functionalized glucose oxidase (GOx+) and horseradish peroxidase, as well as poly(amidoamine) dendrimer-modified horseradish peroxidase, reversibly sorb into polyanionic, hyperbranched poly(sodium acrylate) (PAA-) films… (More)

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@article{Franchina1999ElectrostaticIO, title={Electrostatic immobilization of glucose oxidase in a weak acid, polyelectrolyte hyperbranched ultrathin film on gold: fabrication, characterization, and enzymatic activity.}, author={J G Franchina and William M. Lackowski and Dan L Dermody and Richard M. Crooks and David E. Bergbreiter and Kamalesh K. Sirkar and Robert John Russell and Michael Pishko}, journal={Analytical chemistry}, year={1999}, volume={71 15}, pages={3133-9} }