Electrostatic contributions to the binding of Ca2+ in calbindin D9k.

@article{Linse1991ElectrostaticCT,
  title={Electrostatic contributions to the binding of Ca2+ in calbindin D9k.},
  author={Sara Linse and Carina Johansson and Peter Brodin and Thomas Grundstr{\"o}m and Torbjorn Drakenberg and Sture Fors{\'e}n},
  journal={Biochemistry},
  year={1991},
  volume={30 1},
  pages={154-62}
}
A set of accurate experimental data is provided for Ca2+ ion binding to calbindin D9k, a protein in the calmodulin superfamily of intracellular regulatory proteins. The study comprises both the role of protein surface charges and the effects of added electrolyte. The two macroscopic Ca2(+)-binding constants K1 and K2 are determined for the wild-type and eight mutant calbindins in 0, 0.05, 0.10, and 0.15 M KCl from titrations in the presence of Quin 2 or 5,5'-Br2BAPTA. The mutations involve… CONTINUE READING

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A set of accurate experimental data is provided for Ca2 + ion binding to calbindin D9k , a protein in the calmodulin superfamily of intracellular regulatory proteins .
A set of accurate experimental data is provided for Ca2 + ion binding to calbindin D9k , a protein in the calmodulin superfamily of intracellular regulatory proteins .
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