Electroporation of inositol 1,4,5-triphosphate induces repetitive calcium oscillations in murine oocytes.

@article{Rickords1993ElectroporationOI,
  title={Electroporation of inositol 1,4,5-triphosphate induces repetitive calcium oscillations in murine oocytes.},
  author={Lee F. Rickords and Kenneth L. White},
  journal={The Journal of experimental zoology},
  year={1993},
  volume={265 2},
  pages={178-84}
}
The purpose of these experiments was to determine the effect of electroporation of IP3 into the cytosol of murine secondary oocytes and evaluate any alterations in [Ca2+]i resulting from Ca2+ release from intracellular stores. In addition, we evaluated the effect of ethanol (ETOH) on the release of Ca2+ from intracellular stores. Oocytes were loaded with the Ca2+ indicator fluo-3 by incubation in 100 microliters drops of medium containing 2 microM fluo-3/AM for 60 min at 37 degrees C. Changes… CONTINUE READING