Electroporation of bovine spermatozoa to carry foreign DNA in oocytes.

  title={Electroporation of bovine spermatozoa to carry foreign DNA in oocytes.},
  author={M B Gagn{\'e} and François Pothier and Marc-Andr{\'e} Sirard},
  journal={Molecular reproduction and development},
  volume={29 1},
In the present study, electroporation was used to test the ability of spermatozoa to carry foreign DNA into the bovine oocytes. Frozen-thawed bovine spermatozoa (10(7)/ml) were electroporated using six different combinations of voltage (500, 1,000, or 1,500 V) and capacitance (1 or 25 microFarads) in the presence of 1 mg/ml of plasmid pRGH527. The portions of plasmids retained by sperm cells after three washings (stable for ten washings) were 4.3, 5.5, 5.1, 6.0, 6.8, and 5.8% for 1 microFarad… CONTINUE READING
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