Electroporation-mediated uptake of proteins into mammalian cells.

Abstract

Proteins of up to 230 kilodaltons are taken up by chinese hamster ovary fibroblasts exposed to electroporation under conditions generally similar to those used to mediate DNA transfection. The uptake of catalase, ovalbumin, and histone H1 labelled with fluorescein was visualized by fluorescence microscopy. Under the same conditions, the uptake of colloidal… (More)

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