Electroporation-based technologies for medicine: principles, applications, and challenges.

  title={Electroporation-based technologies for medicine: principles, applications, and challenges.},
  author={Martin L. Yarmush and Alexander Golberg and Gregor Ser{\vs}a and Tadej Kotnik and Damijan Miklav{\vc}i{\vc}},
  journal={Annual review of biomedical engineering},
When high-amplitude, short-duration pulsed electric fields are applied to cells and tissues, the permeability of the cell membranes and tissue is increased. This increase in permeability is currently explained by the temporary appearance of aqueous pores within the cell membrane, a phenomenon termed electroporation. During the past four decades, advances in fundamental and experimental electroporation research have allowed for the translation of electroporation-based technologies to the clinic… 

Figures from this paper

Tutorial: Electroporation of cells in complex materials and tissue

Electroporation is being successfully used in biology, medicine, food processing, and biotechnology, and in some environmental applications. Recent applications also include in addition to classical

Recent advances in microfluidic-based electroporation techniques for cell membranes.

The primary electroporation mechanism is discussed to help establish a clear framework and several critical terms are highlighted to present a better understanding of the theory of aqueous pores within the context of its principles.

The good and the bad of cell membrane electroporation.

The effects of electroporation on the key components of biological membranes, as well as the main applications of this procedure in medicine, such as electrochemotherapy, gene electrotransfer, and tissue ablation are discussed.

The Application of Electric Fields in Biology and Medicine

A field- transduction mechanism that shares features common to the detection of fluid shear by cells is described, and some experimental evidence is provided that supports this model.

Electrodes and Electric Field Distribution in Clinical Practice

Biological effects observed in electroporation on tissue level are described, common electrode designs used in clinical practice are shown and the basic principles of numerical modeling and treatment planning are explained.

Atomistic Simulations of Electroporation of Model Cell Membranes.

  • M. Tarek
  • Biology
    Advances in anatomy, embryology, and cell biology
  • 2017
This chapter provides the reader with a comprehensive account of recent advances in using atomistic modeling in general and molecular dynamics simulations to complement conventional experimental approaches in characterizing several aspects of cell membranes electroporation.

Design of tumor therapy system based on irreversible electroporation

Irreversible electroporation(IRE) based on the high-voltage and steep pulses is a new technique developed in recent years to treat cancer. In this paper, the high-voltage and steep pulses tumor

Electroporation Cuvette with Integrated Electrodes for High Gradient Electric Field Generation

Electroporation is widely used in various biomedical and biotechnological applications, while homogeneous electric fields are usually required for successful treatment. In this work, a model of



Electroporation: theory and methods, perspectives for drug delivery, gene therapy and research.

  • J. Gehl
  • Biology
    Acta physiologica Scandinavica
  • 2003
The theory of electroporation is outlined, the use of short high-voltage pulses to overcome the barrier of the cell membrane is described, and data on safety and efficacy is summed up on delivery of chemotherapy to tumours in cancer patients.

Nonthermal Irreversible Electroporation: Fundamentals, Applications, and Challenges

An overview on the development of NTIRE, its current state-of-the-art, challenges, and future needs is provided.

Irreversible Electroporation: A New Ablation Modality — Clinical Implications

There appears to be complete ablation to the margin of blood vessels without compromising the functionality of the blood vessels, which suggests that IRE is a promising method for treatment of tumors near blood vessels (a significant challenge with current ablation methods).

Flow-through electroporation based on constant voltage for large-volume transfection of cells.

Tissue Ablation with Irreversible Electroporation

It is shown through mathematical analysis that irreversible electroporation can ablate substantial volumes of tissue, comparable to those achieved with other ablation techniques, without causing any detrimental thermal effects and without the need of adjuvant drugs.

Theory of electroporation: A review

Methods of optimization of electrical impedance tomography for imaging tissue electroporation

A method of simultaneously collecting data from all the electrodes, essentially capturing the state of the tissue at a single instant is proposed, which allows a speedy and continuous collection of data, a vital part of real-time electroporation monitoring.

Electroporation of cell membranes.

In vivo imaging of irreversible electroporation by means of electrical impedance tomography

This paper is the first report of a new method for real-time three-dimensional imaging of an in vivo electroporation process and shows good correlation between the extent of tissue damage caused by irreversible Electroporation and the EIT images.

Skin electroporation for transdermal and topical delivery.