Electroporation: theory and methods, perspectives for drug delivery, gene therapy and research.

@article{Gehl2003ElectroporationTA,
  title={Electroporation: theory and methods, perspectives for drug delivery, gene therapy and research.},
  author={Julie Gehl},
  journal={Acta physiologica Scandinavica},
  year={2003},
  volume={177 4},
  pages={
          437-47
        }
}
  • J. Gehl
  • Published 1 April 2003
  • Medicine
  • Acta physiologica Scandinavica
Electroporation designates the use of short high-voltage pulses to overcome the barrier of the cell membrane. By applying an external electric field, which just surpasses the capacitance of the cell membrane, transient and reversible breakdown of the membrane can be induced. This transient, permeabilized state can be used to load cells with a variety of different molecules, either through simple diffusion in the case of small molecules, or through electrophoretically driven processes allowing… 
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  • 2008
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Externally applied voltages can create transient, non-selective pores in a cell's membrane, a phenomenon known as electroporation. Electroporation has reduced toxicity, is easy to perform, and does
What is (Still not) Known of the Mechanism by Which Electroporation Mediates Gene Transfer and Expression in Cells and Tissues
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The focus of this review is to describe the different aspects of what is known of the mechanism of membrane permeabilization and associated gene transfer and, by doing so, what are the actual limits of the DNA delivery into cells.
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An overview of the theory and practice of electroporative gene transfer is provided to aid researchers that wish to employ the method for preclinical and translational gene therapy, NAV, and functional genomic research.
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The physico-chemical theory of ME and electroporative transport in terms of time-dependent flow coefficients has been developed to such a degree that analytical expressions are available to handle curvature and ionic strength effects on ME and transport.
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The results of this study clearly demonstrate that numerical model is reliable and can be very useful in the additional search for electrodes that would make electrochemotherapy and in vivo electroporation in general more efficient and shows that better coverage of tumors with sufficiently high electric field is necessary for improved effectiveness of electroChemotherapy.
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TLDR
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TLDR
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A syringe electrode of this design is described, with which the same transfection efficiency can be achieved by using much lower electric field strength than that of conventional electrodes, such that the tissue damage is minimized.
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A simple physical model for the enhanced DNA penetration into cells in high electric fields is proposed, according to which the interaction of the external electric field with the lipid dipoles of a pore configuration induces and stabilizes the permeation sites and thus enhances cross membrane transport.
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