Electrophysiology, immunophenotype, and gene expression characterization of senescent and cryopreserved human amniotic fluid stem cells

@article{Iordache2016ElectrophysiologyIA,
  title={Electrophysiology, immunophenotype, and gene expression characterization of senescent and cryopreserved human amniotic fluid stem cells},
  author={Florin Iordache and A. C. Constantinescu and Eugen Andrei and Bogdan Amuzescu and Ferdinand Halitzchi and Lorand Savu and Horia Maniu},
  journal={The Journal of Physiological Sciences},
  year={2016},
  volume={66},
  pages={463-476}
}
We characterized human amniotic fluid stem cells (AFSC) in senescent cultures (6 weeks) versus cryopreserved cells using whole-cell patch-clamp, immunophenotyping, and differential gene expression profiling for senescence genes. We evidenced five ion current components (outward rectifier, A-type, inward rectifier, and big conductance Ca2+-dependent K… CONTINUE READING