Electrophysiological studies on primary cultures of proximal tubule cells.

  title={Electrophysiological studies on primary cultures of proximal tubule cells.},
  author={Elsa Bello-Reuss and Mark R. Weber},
  journal={The American journal of physiology},
  volume={251 3 Pt 2},
Primary confluent monolayers were grown from proximal tubule fragments of rabbit kidneys. The fragments were obtained by gradient centrifugation and seeded on an ad hoc dish whose bottom was a permeable and transparent collagen membrane. The culture medium was a mixture of 50% Ham's F-12 and 50% Dulbecco's modified Eagle's medium supplemented with insulin, transferrin, ethanolamine, sodium selenite, and amino acids. The monolayers were studied at 6-14 days after seeding. Transmission electron… CONTINUE READING


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