Electrophysiological studies in principal cells of rat cortical collecting tubules ADH increases the apical membrane Na+-conductance

@article{Schlatter2004ElectrophysiologicalSI,
  title={Electrophysiological studies in principal cells of rat cortical collecting tubules ADH increases the apical membrane Na+-conductance},
  author={E. Schlatter and J. Schafer},
  journal={Pfl{\"u}gers Archiv},
  year={2004},
  volume={409},
  pages={81-92}
}
The mechanism of ion transport across principal cells of rat cortical collecting tubules (CCT) and its regulation by vasopressin (ADH) has been studied in the isolated perfused tubule. To amplify the response to ADH rats were treated with 5 mg I. M. desoxycorticosterone 4–9 days prior to the experiments. Addition of 2·10−10 mol·1−1 ADH increased the transepithelial voltage from −5.1 ±0.7 mV to −16.1±1.4 mV (n=37) and decreased the transepithelial resistance from 51±4 Ω cm2 to 39±2 Ω cm2 (n=33… Expand
Principal cells of cortical collecting ducts of the rat are not a route of transepithelial Cl− transport
TLDR
It is concluded that acellCl of principal cells in the rat CCD is not far above passive equilibrium, and that these cells do not contribute significantly to transepithelial Cl− reabsorption, which must occur by alternative routes such as the paracellular pathway, and/or through intercalated cells. Expand
Ionic conductances of cultured principal cell epithelium of renal collecting duct
TLDR
The studies of a pure principal cell epithelium confirm findings described for the intact cortical collecting duct and add new information concerning chloride conductivity and related blocking agents. Expand
Electrophysiological study of inner medullary collecting duct of hamsters
TLDR
Findings are compatible with the view that either a weak Na+-K+ pump or an ouabain-resistant pump in combination with high K+ conductance in the basolateral membrane mainly accounts for the maintenance of the intracellular concentration of Na+ and K+. Expand
Na/Ca exchangers in collecting cells of rat kidney
TLDR
The data suggest the presence of 3 Na+/1 Ca2+ exchangers in cell membranes of rat CCT and the calcium flux equation derived by Läuger for the exchanger indicates a non-linear relationship between net calcium flux and driving force which could account for the difference observed here between the poor effect of applying either low [Na+]o or ouabain alone and the large peak of [Ca2+]i induced by combining these two conditions. Expand
Activity of the basolateral K+ channels is coupled to the Na+-K+-ATPase in the cortical collecting duct.
TLDR
It is concluded that stimulation of Na+-K+-ATPase increases the basolateral K+ conductance and that this effect involves suppression of nitric oxide-dependent inhibition of K+ channels. Expand
K+ channels in the basolateral membrane of rat cortical collecting duct
TLDR
The high activity of both K+ channels of the basolateral membrane on the cell indicates that they may serve for K+ recirculation across the basosolic side of the membrane. Expand
pH dependence of K+ conductances of rat cortical collecting duct principal cells
TLDR
It is concluded that cellular acidification in rat CCD principal cells down-regulates K+ conductances, thus reduces K+ secretion by direct inhibition of K+ channel activity. Expand
Na channels in the rat connecting tubule.
  • G. Frindt, L. Palmer
  • Chemistry, Medicine
  • American journal of physiology. Renal physiology
  • 2004
TLDR
Quantitative analysis of the results suggests that the maximal capacity of the aggregate population of CNTs to reabsorb Na could be as high as 18 micromol/min, or approximately 10% of the filtered load of Na, approximately 10 times higher than that of the CCT. Expand
Activation of the epithelial Na+ channel in the collecting duct by vasopressin contributes to water reabsorption.
TLDR
A quantitatively important action of vasopressin is demonstrated on ENaC suggesting that distal nephron Na+ re absorption mediated by this channel contributes to maintenance of water reabsorption. Expand
Ion conductances of isolated cortical collecting duct cells
TLDR
These freshly isolated CCD cells seem to be a powerful preparation to study the properties and regulation of ion conductances of rat CCD with several electrophysiological methods. Expand
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