Electrophysiological studies in principal cells of rat cortical collecting tubules ADH increases the apical membrane Na+-conductance

@article{Schlatter1987ElectrophysiologicalSI,
  title={Electrophysiological studies in principal cells of rat cortical collecting tubules ADH increases the apical membrane Na+-conductance},
  author={Eberhard Schlatter and James A. Schafer},
  journal={Pfl{\"u}gers Archiv},
  year={1987},
  volume={409},
  pages={81-92},
  url={https://api.semanticscholar.org/CorpusID:24655136}
}
It is concluded that ADH stimulates Na+ reabsorption and K+ secretion in the rat CCT primarily by increasing the Na+ conductance in the apical cell membrane.
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82 Citations

Principal cells of cortical collecting ducts of the rat are not a route of transepithelial Cl− transport

It is concluded that acellCl of principal cells in the rat CCD is not far above passive equilibrium, and that these cells do not contribute significantly to transepithelial Cl− reabsorption, which must occur by alternative routes such as the paracellular pathway, and/or through intercalated cells.

Ionic conductances of cultured principal cell epithelium of renal collecting duct

The studies of a pure principal cell epithelium confirm findings described for the intact cortical collecting duct and add new information concerning chloride conductivity and related blocking agents.

Electrophysiological study of inner medullary collecting duct of hamsters

Findings are compatible with the view that either a weak Na+-K+ pump or an ouabain-resistant pump in combination with high K+ conductance in the basolateral membrane mainly accounts for the maintenance of the intracellular concentration of Na+ and K+.

Na/Ca exchangers in collecting cells of rat kidney

The data suggest the presence of 3 Na+/1 Ca2+ exchangers in cell membranes of rat CCT and the calcium flux equation derived by Läuger for the exchanger indicates a non-linear relationship between net calcium flux and driving force which could account for the difference observed here between the poor effect of applying either low [Na+]o or ouabain alone and the large peak of [Ca2+]i induced by combining these two conditions.

Activity of the basolateral K+ channels is coupled to the Na+-K+-ATPase in the cortical collecting duct.

It is concluded that stimulation of Na+-K+-ATPase increases the basolateral K+ conductance and that this effect involves suppression of nitric oxide-dependent inhibition of K+ channels.

K+ channels in the basolateral membrane of rat cortical collecting duct

The high activity of both K+ channels of the basolateral membrane on the cell indicates that they may serve for K+ recirculation across the basosolic side of the membrane.

pH dependence of K+ conductances of rat cortical collecting duct principal cells

It is concluded that cellular acidification in rat CCD principal cells down-regulates K+ conductances, thus reduces K+ secretion by direct inhibition of K+ channel activity.

Na channels in the rat connecting tubule.

Quantitative analysis of the results suggests that the maximal capacity of the aggregate population of CNTs to reabsorb Na could be as high as 18 micromol/min, or approximately 10% of the filtered load of Na, approximately 10 times higher than that of the CCT.

Activation of the epithelial Na+ channel in the collecting duct by vasopressin contributes to water reabsorption.

A quantitatively important action of vasopressin is demonstrated on ENaC suggesting that distal nephron Na+ re absorption mediated by this channel contributes to maintenance of water reabsorption.

Ion conductances of isolated cortical collecting duct cells

These freshly isolated CCD cells seem to be a powerful preparation to study the properties and regulation of ion conductances of rat CCD with several electrophysiological methods.
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44 References

Effects of ouabain and temperature on cell membrane potentials in isolated perfused straight proximal tubules of the mouse kidney

The present data indicate that the contribution of peritubular potassium conductance to the cell membrane conductance decreases following inhibition of basolateral (Na++K-)-ATPase, and cooling from 37° to 10°C does not only reduce (Na−+K+)-ATpase activity but in addition luminal sodium uptake mechanisms such as the sodium glucose cotransporter.

cAMP increases the basolateral Cl−-conductance in the isolated perfused medullary thick ascending limb of Henle's loop of the mouse

It is proposed that stimulation of active NaCl re absorption in the mTAL segment of the mouse by ADH, mediated via cAMP, increases primarily the basolateral chloride conductance.

Effect of ADH on rubidium transport in isolated perfused rat cortical collecting tubules.

The results indicate that ADH augments net K+ secretion either by an increase in the Ba2+-sensitive conductance of the apical membrane or by an increased in the electrochemical potential driving force for net Rb+ secretion through this pathway.

Sustained response to vasopressin in isolated rat cortical collecting tubule.

The higher water permeabilities are consistent with the shorter length of the cortical collecting tubule in the rat, and may reflect the importance of attaining osmotic equilibration within the cortex during maximal antidiuresis.

Electrophysiological properties of cellular and paracellular conductive pathways of the rabbit cortical collecting duct

It is concluded that both the transcellular and paracellular pathways of the cortical collecting tubule have high ionic conductances, and that the apical membrane conductance primarily reffects a high potassium conductance.

Potassium transport in cortical collecting tubules from mineralocorticoid-treated rat.

The results indicate either that the concentrations of Ba2+ used were insufficient to block K+ conductances completely or that K+/Rb+ secretion can occur through a Ba2-insensitive pathway.

Characterization of apical cell membrane Na+ and K+ conductances of cortical collecting duct using microelectrode techniques.

It is concluded that microelectrode techniques can be applied successfully to the cortical collecting duct and that the apical cell membrane possesses an amiloride-sensitive Na+ conductance and a Ba2+- and H+-sensitive K+ Conductance.

Sodium transport by rat cortical collecting tubule. Effects of vasopressin and desoxycorticosterone.

The facilitating action of ADH on DOCA-induced stimulation of sodium transport may be important for maximal sodium reabsorption and for the ability to achieve a maximally concentrated urine.

Cellular electrophysiology of potassium transport in the mammalian cortical collecting tubule

Electrophysiological studies were carried out on single perfused cortical and medullary collecting ducts to define the potassium and sodium transport properties of their apical and basolateral cell…

Cation selectivity of the isolated perfused cortical thick ascending limb of Henle's loop of rabbit kidney

    R. Greger
    Biology, Medicine
    Pflügers Archiv
  • 2004
The cortical thick ascending limb of Henle's loop has a low resistance pathway which is cation selective similar to that of “leaky” epithelia, and since the membrane diffusion potentials are symmetric and since they are not altered by inhibition of active transport, it is likely that thisLow resistance pathway is formed by a paracellular shunt.