Electrophoretically mediated microanalysis with partial filling technique and indirect or direct detection as a tool for inhibition studies of enzymatic reaction.

@article{Telnarov2004ElectrophoreticallyMM,
  title={Electrophoretically mediated microanalysis with partial filling technique and indirect or direct detection as a tool for inhibition studies of enzymatic reaction.},
  author={Magdal{\'e}na Telnarov{\'a} and Soňa Vytiskov{\'a} and Marta Monincov{\'a} and Zdeněk Glatz},
  journal={Electrophoresis},
  year={2004},
  volume={25 7-8},
  pages={1028-33}
}
The inhibition of the model enzyme, haloalkane dehalogenase from Sphingomonas paucimobilis, was investigated by a combination of electrophoretically mediated microanalysis with a partial filling technique, followed by indirect or direct detection. In this setup, part of the capillary is filled with a buffer suitable for the enzymatic reaction (20 mM glycine buffer, pH 8.6) whereas the rest of the capillary is filled with the background electrolyte optimal for separation of substrates and… CONTINUE READING