Electrophoretic separation of proteins via complexation with a polyelectrolyte
@article{Baskin2002ElectrophoreticSO, title={Electrophoretic separation of proteins via complexation with a polyelectrolyte}, author={E. M. Baskin and B. I. Shklovskii and Gleb Zilberstein}, journal={Physica A-statistical Mechanics and Its Applications}, year={2002}, volume={317}, pages={313-320} }
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Parallel isoelectric focusing II
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It is shown that the method guarantees the fast and complete separation of any complex protein mixtures under acceptable conditions, such as voltage source, temperature, size of the device, and separation process duration.
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Through physical chemistry parameters, free energies of interactions, and the mapping of amino acid pKa shifts and polyelectrolyte trajectories, the charge regulation mechanism is shown to be the most important contributor in protein-polyelectrolete complexation regardless of pH, dipole moment, and protein capacitance in a low salt regime.
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