Electrophoretic purification of tumor-targeted polyethylenimine-based polyplexes reduces toxic side effects in vivo.

@article{Fahrmeir2007ElectrophoreticPO,
  title={Electrophoretic purification of tumor-targeted polyethylenimine-based polyplexes reduces toxic side effects in vivo.},
  author={Julia Fahrmeir and Michael Gunther and Nicole Tietze and Ernst Wagner and Manfred Ogris},
  journal={Journal of controlled release : official journal of the Controlled Release Society},
  year={2007},
  volume={122 3},
  pages={236-45}
}
Non-viral vectors based on polyethylenimine (PEI) are usually generated with an excess of PEI. However, the amount of unbound polymer correlates with toxicity limiting the in vivo use of these gene carriers. Purification based on size exclusion chromatography of PEI/DNA polyplexes smaller than 200 nm has been shown to efficiently remove unbound PEI polymer. A novel purification method based on electrophoresis can purify PEI polyplexes independent of their size resulting in polyplexes with final… CONTINUE READING

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