Electrophoretic mobility shift assay for the detection of specific DNA-protein complex in nuclear extracts from the cultured cells and frozen autopsy human brain tissue.

@article{Lahiri2000ElectrophoreticMS,
  title={Electrophoretic mobility shift assay for the detection of specific DNA-protein complex in nuclear extracts from the cultured cells and frozen autopsy human brain tissue.},
  author={Debomoy K. Lahiri and Yuk Wah Wong Ruifang Ge},
  journal={Brain research. Brain research protocols},
  year={2000},
  volume={5 3},
  pages={257-65}
}
The electrophoretic mobility shift assay (EMSA) is generally used to study the interaction of transcription factors to specific DNA sequences. The preparation of high quality nuclear extracts is an important step before performing the assay. Here we describe a rapid method for the isolation of good-quality DNA-binding proteins from cultured cell lines and autopsy tissue samples from the human brain. The 'rapid method' (RM) utilizes the low salt/detergent lysis steps followed by high salt… CONTINUE READING

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