Electrophoretic fractionation of pancreatic lipase.

@article{Panteghini1992ElectrophoreticFO,
  title={Electrophoretic fractionation of pancreatic lipase.},
  author={Mauro Panteghini},
  journal={Clinical chemistry},
  year={1992},
  volume={38 9},
  pages={
          1712-6
        }
}
  • M. Panteghini
  • Published 1 September 1992
  • Chemistry, Medicine
  • Clinical chemistry
Human pancreatic isolipases were separated by electrophoresis on cellulose acetate membrane. Band visualization by fluorescence detection of NADH produced by beta-oxidation cyclic reaction of fatty acid liberated from 1,2-dilinoleoylglycerol by lipase was achieved by making a two-plate "sandwich" of an electrophoresis plate and a substrate-bearing plate. Two true lipase forms, named L1 and L2 to reflect their electrophoretic migration, were resolved with this method and quantified by… 
The never-ending search of an acceptable compromise for pancreatic lipase standardisation
  • M. Panteghini
  • Medicine
    Clinical chemistry and laboratory medicine
  • 2012
TLDR
An optimised titrimetric procedure at constant pH for HPL using triolein-based emulsion as substrate, a standardised mode of substrate emulsifi cation, and optimisation of HPL effectors, such as sodium deoxycholate, calcium ions and colipase are developed.
Lipase in serum--the elusive enzyme: an overview.
TLDR
The clinical sensitivity of all modern assays is high because of selection of a low decision limit; the clinical specificity varies greatly but can be improved by increasing the cutoff point; and the clinical utility of lipase determinations is summarized.
Electrophoresis on cellulose acetate and cellogel: current status and perspectives
Abstract After introducing some general concepts of cellulose acetate membranes (CAMs) and gelled cellulose acetate membranes (GCAMs and their use for electrophoretic separations, an overview on CAM-
Chronic increased serum lipase without evidence of pancreatitis: tumor-derived lipase?
TLDR
A 51-year-old man developed a large retroperitoneal tumor with liver and lymph node metastases; there was no radiological evidence of pancreatic involvement; the source of this increased serum lipase activity was the nonpancreatic tumor.
Chronic increased serum lipase in a patient with suspected pancreatic adenocarcinoma.
TLDR
A 57-year-old man presented with cutaneous lesions in his legs, characterized by lobular panniculitis with fat necrosis and ghost cells, and high lipase activity led us to consider a pancreatic carcinoma with liver metastasis with papillary differentiated adenocarcinoma.
Valoración de isoamilasas e isolipasas en el suero de pacientes con insuficiencia renal
TLDR
The combined study of serum levels of isoamylases and isolipases could be an effective marker for diagnosis and evolution of associated pancreatitis with acute or chronic renal failure.
Pancreatic hyperenzymemia: clinical significance and diagnostic approach.
TLDR
This review emphasizes the biological mechanisms behind serological alterations of pancreatic enzymes, the possible causes, the clinical implications and the diagnostic approach.
Serum amylase and lipase and urinary trypsinogen and amylase for diagnosis of acute pancreatitis.
TLDR
The sensitivity, specificity, post-test probability, and specificities of the index tests in people presenting to the emergency department with acute abdominal pain are calculated and reported along with 95% confidence interval (CI) for each cutoff.
Automated analysis of electrophoretic gels by image digitalization and processing
TLDR
A system and method of automated analysis of electrophoresis gels by image digitalization and processing are reported, formed by a non-intensified colour charge-coupled device camera interfaced to a microcomputer through a frame grabber that allows image acquisition to be performed in real time.
Pancreatic lipase: why laboratory community does not take enough care of this clinically important test?
TLDR
Important issues that, if not correctly managed and solved, make misdiagnosis of acute pancreatitis by using serum LIP a real possibility are discussed.
...
1
2
...

References

SHOWING 1-10 OF 15 REFERENCES
Lipase isoforms and amylase isoenzymes: assays and application in the diagnosis of acute pancreatitis.
Pancreatic juice and serum from patients with acute pancreatitis contain three enzymes that have lipolytic activity: L1 and L2, which are pancreatic isoenzymes or isoforms of lipase (EC 3.1.1.3), and
Differentiating two forms of plasma lipase by use of media with different ionic strengths.
TLDR
Three different techniques of lipase determination used to investigate an interesting effect of ionic strength on enzyme activity found that pancreatitis-type lipase was activated by 140 mmol/L NaCl solution only if colipase and sodium deoxycholate were also present, which suggests that these components are cofactors in the activating effect.
Assays of serum lipase: analytical and clinical considerations.
TLDR
Serum lipase determinations with the current, simpler technology are superior to total amylase in the diagnosis of patients with acute pancreatitis because of the presence of the necessary cofactor, colipase, in their reagents.
Human pancreatic lipase: a glycoprotein.
TLDR
Immunological cross reactions have been obtained between human lipase and other mammalian lipases (porcine, bovine, ovine, canine and rat) and both are glycoproteins.
Diagnostic value of measuring pancreatic lipase and the P3 isoform of the pancreatic amylase isoenzyme in serum of hospitalized hyperamylasemic patients.
TLDR
P3 isoform and LPS appear to be interchangeable markers of pathological release of pancreatic enzymes into the bloodstream during acute pancreatitis, which decreases the need for the expensive radiological procedures currently required to confirm this diagnosis.
A rapid and sensitive turbidimetric method for serum lipase based upon differences between the lipases of normal and pancreatitis serum.
TLDR
The serum of patients with acute pancreatitis contains a lipase with optimal action at pH 9.1 and sodium deoxycholate concentration of 0.35%.
Macro Lipase — A New Member of the Family of Immunoglobulin-Linked Enzymes
  • W. Stein, J. Bohner, M. Bahlinger
  • Biology, Medicine
    Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie
  • 1987
TLDR
Results of exclusion chromatography and immunological investigations clearly demonstrate that the atypical time-course of lipase activity is not due to injury of the pancreas or alterations of the patient's lipase, but rather due to the presence oflipase-binding autoantibodies, resulting in the formation of immune complexes with high molecular mass between lipase and immunoglobulin G lambda.
Plasma lipase properties as related to pancreatic condition.
TLDR
Gel-filtration experiments suggest that the two forms of lipase in plasma have different relative molecular masses; moreover, their avidity for antibodies against human pancreatic lipase differs.
Laboratory tests in the differential diagnosis of hyperamylasemia.
TLDR
The method for pancreatic-type isoamylase based on selective inhibition is satisfactory for routine clinical laboratory use and a normal result tends to exclude acute pancreatitis.
Lipase activity in serum measured with Ektachem is often increased in nonpancreatic disorders.
TLDR
Serum lipase activity measured with the Ektachem assay is also often increased in patients with intra-abdominal disorders that appear to be nonpancreatic, while serum lipase values greater than URL had 100% sensitivity and 84% specificity.
...
1
2
...