Electrophoretic detection of population variation within Contracaecum ogmorhini (Nematoda: Ascaridoidea: Anisakidae).

@article{Zhu2001ElectrophoreticDO,
  title={Electrophoretic detection of population variation within Contracaecum ogmorhini (Nematoda: Ascaridoidea: Anisakidae).},
  author={Xing Quan Zhu and Stefano D'Am{\'e}lio and Min Hu and Lia Paggi and Robin B. Gasser},
  journal={Electrophoresis},
  year={2001},
  volume={22 10},
  pages={
          1930-4
        }
}
This study examined genetic variation among specimens of Contracaecum ogmorhini from different otariid hosts and geographical origins using a polymerase chain reaction (PCR)-based mutation detection approach. The first (ITS-1) and second (ITS-2) internal transcribed spacers (ITS) of ribosomal DNA (rDNA) were amplified individually by PCR, scanned for sequence variation by single-strand conformation polymorphism (SSCP), and samples displaying variable SSCP profiles were subjected to cycle… CONTINUE READING
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