Electrophoretic characterization of pyrimidine 5'-nucleotidase of human erythrocytes and its distinction from acid phosphatase.


Electrophoresis of red cell pyrimidine 5'-nucleotidase was carried out on cellulose acetate strips. One major band of activity was found in preparations from human erythrocytes. This enzyme showed a specificity for the pyrimidine nucleotides UMP and CMP. No activity was detected with AMP. Pyrimidine 5'-nucleotidase activity could be separated from that of… (More)


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